šídák post hoc test  (GraphPad Software Inc)

Journal: Autophagy

Article Title: Autophagy deficiency leads to accumulation of ubiquitinated proteins, ER stress, and cell death in Arabidopsis

doi: 10.4161/auto.29406

Figure Lengend Snippet: Figure 1. NPR1 is not required for the promotion of HR cell death. ( A and B ) Ion leakage assays of ( A ) 6-wk-old Col-0 WT, atg5, npr1, and atg5 npr1 plants and ( B ) 6-wk-old Col-0 WT, rpm1 , atg2, npr1, and atg2 npr1 plants after inoculation with 1 x 10 8 CFU mL −1 Pst DC3000 (AvrRpm1). Mean and standard errors (SE) were calculated from 4 or 6 discs per treatment with 3 or 4 replicates within an experiment. Experiments were repeated with similar results. Pairwise comparisons at the last time point post infection for means vs. WT were performed with the one-way ANOVA test followed by the Holm-Šídák post-hoc test. a, P ≤ 0.05, b, P ≤ 0.01, c, P ≤ 0.0001. ( C ) Evaluation of tissue viability using lactophenol-trypan blue staining in leaves from Col-0 WT, npr1, atg2, atg2 npr1, and rpm1, 12 h after infection with 1 x 10 6 CFU mL −1 Pst DC3000 (AvrRpm1). Dead cells (labeled blue) are observed in leaves of all genotypes (except rpm1) treated with Pst DC3000 (AvrRpm1). No dead cells were observed in leaves infiltrated with MgCl 2 (negative control) or in rpm1 mutants which are unable to trigger HR in the presence of the AvrRpm1 effector. Size bar: 2.5 mm.

Article Snippet: Statistical analyses were performed with an ANOVA with the post-hoc Holm-Šídák test, using GraphPad Prism 6.

Techniques: Infection, Staining, Labeling, Negative Control